Table 1 Binding kinetics of the isolated IL-4Rα Abs to IL-4Rαa.

From: Engineering of anti-human interleukin-4 receptor alpha antibodies with potent antagonistic activity

 

IgG Abs

kon (1/M s)

koff (1/s)

KD (M)

R 2

Initial screening

Dupilumab analogue

(2.99 ± 0.02) × 106

(2.74 ± 0.33) × 10−5

(9.16 ± 1.11) × 10−12

0.99

4 R25

(8.43 ± 0.16) × 105

(1.99 ± 0.03) × 10−3

(2.36 ± 0.06) × 10−9

0.95

4 R34

(3.47 ± 0.01) × 106

(3.61 ± 0.05) × 10−3

(1.04 ± 0.04) × 10−9

0.90

1st affinity maturation

4R34.1

(4.28 ± 0.05) × 106

(1.72 ± 0.01) × 10−3

(4.01 ± 0.06) × 10−10

0.96

4R34.2

(4.70 ± 0.09) × 106

(4.01 ± 0.02) × 10−3

(8.53 ± 0.02) × 10−10

0.94

4R34.19

(4.58 ± 0.08) × 106

(2.43 ± 0.01) × 10−3

(5.32 ± 0.10) × 10−10

0.96

4R34.29

(5.18 ± 0.11) × 106

(3.71 ± 0.03) × 10−3

(7.16 ± 0.16) × 10−10

0.95

2nd affinity maturation

4R34.1.11

(4.62 ± 0.05) × 106

(9.68 ± 0.06) × 10−4

(2.10 ± 0.03) × 10−10

0.98

4R34.1.13

(5.12 ± 0.05) × 106

(1.07 ± 0.06) × 10−3

(2.08 ± 0.02) × 10−10

0.98

4R34.1.17

(4.92 ± 0.06) × 106

(8.79 ± 0.07) × 10−4

(1.79 ± 0.02) × 10−10

0.97

4R34.1.18

(6.05 ± 0.09) × 106

(1.75 ± 0.06) × 10−3

(2.90 ± 0.04) × 10−10

0.96

4R34.1.19

(4.93 ± 0.06) × 106

(8.77 ± 0.06) × 10−4

(1.78 ± 0.02) × 10−10

0.98

4R34.1.21

(4.63 ± 0.04) × 106

(1.06 ± 0.05) × 10−3

(2.28 ± 0.02) × 10−10

0.99

  1. aBinding kinetic of interactions between soluble IL-4Rα and immobilized anti-IL-4Rα Abs, as measured by bio-layer interferometry. The association rate constant (kon), dissociation rate constant (koff), equilibrium dissociation constant (KD), and estimate of the goodness of the curve fit (R2) were obtained using the Octet Data Analysis software (ForteBio). IgG, immunoglobulin G.
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