Figure 8
From: In vitro NTPase activity of highly purified Pdr5, a major yeast ABC multidrug transporter

Test for adenylate kinase activity of Pdr5 as described in37. The relative activity in percent corresponds to the formation of ADP as a function of NADH consumption in an enzyme coupled assay. An increasing amount of AMP at saturating ATP concentration (4 mM) was added and in case of adenylate kinase activity, an increase in activity should be observerd. Error bars represent 3 independent measurements.