Figure 5
From: CGRP signalling inhibits NO production through pannexin-1 channel activation in endothelial cells
Activation of capsaicin-sensitive sensory nerves leads to CGRP receptor-mediated Panx-1-formed channel opening. (A) Experimental protocol and representative images of ethidium (Et) uptake observed during vehicle or 1 µM capsaicin (Caps) application for 20 min in mesenteric resistance arteries in the absence and presence of 1 mM probenecid (Prob), a Panx-1 blocker. (B) Representative images and densitometric analysis of Et uptake attained during the stimulation for 20 min with the vehicle of capsaicin or capsaicin in the absence and presence of 300 nM CGRP8–37, 10 µM BIBN4096, 1 mM probenecid (Prob), 200 µM La3+ and 10 µM PPADS. (C) Capsaicin-induced Et uptake observed in control (Sham-operated rats) and denervated mesenteric arteries. Changes in ethidium-fluorescence signal are expressed in arbitrary units (AU). Numbers inside the bars or in parentheses indicate the n value. Values are means ± SEM. *P < 0.05 vs caps (B) or vehicle (C) by one-way ANOVA plus Dunnett post hoc test. Scale bars represent 100 µm.
