Figure 2
From: Stress-induced tunneling nanotubes support treatment adaptation in prostate cancer
TNTs form between stressed and non-stressed cells. (A) Schema illustrating co-culture assays for stressed and non-stressed LNCaP cells. (B) Images of TNT formation between co-cultured populations: stressed to stressed cells (green to green), non-stressed to non-stressed cells (red to red), non-stressed to stressed cells (red to green) and stressed to non-stressed cells (green to red). (C) TNT formation between co-cultured populations was quantified; percentage of each category is shown. *p < 0.05. (D) DiO-labeled LNCaP cells (green) and Dil-labelled hFOB cells (red) were co-cultured in CSS-containing medium for 24 hours. Image shows a TNT from a LNCaP cell (green) towards an osteoblast cell (red). Scale bar: 20 µm.
