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Figure 1

From: Author Correction: The MFS efflux pump EmrKY contributes to the survival of Shigella within macrophages

Figure 1

Relative efflux pumps encoding gene transcription during S. flexneri infection of (A) U937 and (B) Caco-2. Quantitative analysis of 14 efflux pump transcripts was performed by means of Q-Real Time PCR assay. Total RNA was extracted from intracellular S. flexneri M90T bacteria at various time points p.i., from 0 h (corresponding to bacterial adhesion to the target cells, see MM) up to 3 h. Each infection was repeated three times and at least three wells were run for each sample. The x axis indicates the expression fold-change (RQ value) for each gene on a logarithmic scale. The results are shown relative to the expression of each gene in bacteria grown in RPMI (A) or DMEM (B) set to 1.00. Statistical significance was determined by a one-tailed ANOVA, and p values are as follows: *p < 0.05, **p < 0.01. Error bars represent SD.

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