Figure 4

Bone quality parameters obtained from Raman spectra. (a) Transmission electron microscopy images showing the site used for the mapped image and the mapped images of bone quality parameters. The site used for mapping, with spectra obtained at intervals of 10 µm within the 120 µm × 300 µm area, including the drilled hole. The mapped images of the mandible were colored based on the value of bone quality parameters from the drilled hole to the newly formed bone in OVX and PTH rats. Measured bone quality parameters were mineral/matrix ratio, full-width at half-height (FWHH) of the v₁PO₄ band (indicator of crystallinity), monohydrogen phosphate content, B-type carbonate substitution, and collagen structural integrity. In these mapped images, blue indicates a lower value and red indicates a higher value. Compared to OVX rats, PTH rats showed a decrease in the mineral/matrix ratio and collagen structural integrity, and an increase in the FWHH of the v₁PO₄ band and B-type carbonate substitution. (b,c) Bone parameters obtained from Raman spectra. Similar to those for the mapped images, measured parameters at 90 μm (b) and 2 mm (c) from the bone edge were the mineral/matrix ratio, crystallinity, B-type carbonate substitution, monohydrogen phosphate content, and collagen structural integrity in OVX and PTH rats. *P < 0.05.