Table 2 Nucleotide sequences and base compositions of the MiEel forward primer (MiEel-F) and reverse primer (MiEel-R).
From: New PCR primers for metabarcoding environmental DNA from freshwater eels, genus Anguilla
MiEel-F | 5′- | C | T | T | A | C | A | G | C | A | A | A | C | C | T | G | A | C | A | G | C | A | G | −3′ |  |  |  |  |
| Â | A | 0 | 0 | 0 | 19 | 0 | 19 | 0 | 0 | 18 | 19 | 19 | 0 | 0 | 0 | 9 | 19 | 0 | 19 | 0 | 0 | 19 | 0 | Â | Â | Â | Â | Â |
| Â | C | 19 | 0 | 1 | 0 | 19 | 0 | 0 | 19 | 0 | 0 | 0 | 18 | 19 | 0 | 0 | 0 | 19 | 0 | 0 | 19 | 0 | 0 | Â | Â | Â | Â | Â |
| Â | G | 0 | 0 | 0 | 0 | 0 | 0 | 19 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 10 | 0 | 0 | 0 | 19 | 0 | 0 | 19 | Â | Â | Â | Â | Â |
| Â | T | 0 | 19 | 18 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 19 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | Â | Â | Â | Â | Â |
MiEel-R | 5′- | T | T | G | G | T | G | T | G | C | C | A | T | T | A | T | A | C | G | T | T | T | T | C | T | T | G | −3′ |
| Â | A | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 19 | 0 | 0 | 19 | 0 | 19 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | Â |
| Â | C | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 19 | 19 | 0 | 0 | 0 | 0 | 0 | 0 | 19 | 0 | 0 | 0 | 0 | 0 | 19 | 0 | 0 | 0 | Â |
| Â | G | 0 | 0 | 19 | 19 | 0 | 19 | 0 | 19 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 19 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 19 | Â |
| Â | T | 19 | 19 | 0 | 0 | 19 | 0 | 19 | 0 | 0 | 0 | 0 | 19 | 19 | 0 | 19 | 0 | 0 | 0 | 19 | 19 | 19 | 19 | 0 | 19 | 19 | 0 | Â |
