Figure 4

Adoptive transfer of in vitro-generated MDSCs activates TxT-induced T cells without inducing exhaustion. Mice were untreated or adoptively transferred with MDSCs and subsequently received TxT. (A) 7 days later, splenocytes were cultured for 24 h and CD4⁺ and CD8⁺ T cells were stained for markers defining naïve and activated cells. (B) At day 7 after TxT, CFSE-labelled splenocytes were cultured in medium and 4 days later proliferation of CD3⁺ T cells was analyzed. (C) 7 days after TxT, splenocytes were cultured for 24 h and expression of exhaustion markers was defined on CD4⁺ and CD8⁺ T cells. **P≤0.01, ***P≤0.001 Data represent the mean value ± SD of 8–10 animals/group (A) and 13–14 mice/group (B). (C) FACS staining’s show the expression of markers for 3 mice/group and is representative for one experiment out of 2 experiments performed. Significance was calculated by Student’s t-test.