Figure 2 | Scientific Reports

Figure 2

From: Improved TGIRT-seq methods for comprehensive transcriptome profiling with decreased adapter dimer formation and bias correction

Figure 2

Bioanalyzer traces of TGIRT-seq libraries constructed from varying amounts of different-sized RNA oligonucleotides using either the NTC or NTT adapter. TGIRT-seq libraries were prepared from (A) 40-nt or (B) 20-nt RNA oligonucleotides using the workflow of Fig. 1A. After PCR for 12 cycles and one round of 1.4X AMPure beads clean-up, the libraries were analyzed on a 2100 Bioanalyzer (Agilent) using a high sensitivity DNA chip. M: internal markers.

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