Figure 3 | Scientific Reports

Figure 3

From: Generation of a fully erythromycin-sensitive strain of Clostridioides difficile using a novel CRISPR-Cas9 genome editing system

Figure 3

Colony PCR screening of putative 630Δerm* strains. (A) Diagrammatic representation of the 9.6 kb C. difficile 630 Tn5398 mobilizable transposon sequence. The erm (B) genes are shown in green, other named ORFs shown in purple, and gene remnants and pseudogenes are shown in grey. Red brackets denote the genomic region deleted in 630Δerm, flanked by the left- (LDS) and right-deletion sites (RDS), and targeted for deletion in the generation of 630Δerm*. Schematic binding sites of screening PCR primers Tn5398_sF1 and Tn5398_sR1 (not to scale) and the size of the expected wild-type product are also shown. (B) Colony PCR screening of 25 putative 630Δerm* mutants using Tn5398_sF1 and Tn5398_sR1 primers. ‘M’ denotes DNA marker, ‘dH2O’ denotes negative control lane.

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