Figure 4 | Scientific Reports

Figure 4

From: Generation of a fully erythromycin-sensitive strain of Clostridioides difficile using a novel CRISPR-Cas9 genome editing system

Figure 4

Diagrammatic representation of trCas9 sequence and annotated features. Top: Domain organisation within wild-type S. pyogenes Cas9. Bottom: Domain organisation and DNA sequence of our trCas9 and upstream thiolase promoter (Pthl) from pMTL431511-CLAU-pyrE. The frameshift-generating adenine base insertion at position 130 within the cas9 RuvCI domain, which results in a truncated polypeptide of 53 amino acids, is indicated in capital letters. Putative ribosome binding site and downstream trCas9 ORF lacking 87 amino acids from the N-terminal end of Cas9 also indicated.

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