Figure 5 | Scientific Reports

Figure 5

From: Generation of a fully erythromycin-sensitive strain of Clostridioides difficile using a novel CRISPR-Cas9 genome editing system

Figure 5

Colony PCR screening of putative 630Δerm*ΔpyrE strains. Top: Diagrammatic representation of the C. difficile 630 chromosomal region containing pyrE, including schematic binding sites of screening primers 630_pyrD_sF1 and 630_0189_sR3 (not to scale). Bottom: Agarose gel electrophoresis following colony PCR screens for the desired 234 bp truncation of pyrE following initial streaking of thiamphenicol resistant transconjugant colonies to purity (A) and two subsequent colony PCR screens following an additional one (B), or two (C), rounds of passaging on BHIS agar supplemented with thiamphenicol. ‘M’ denotes DNA marker, ‘dH2O’ denotes negative control lane.

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