Figure 6

Phenotypic characterisation of NCTC 630 and 630Δerm*ΔpyrE strains. Growth of C. difficile strains NCTC 630 (N) and three independently generated 630Δerm*ΔpyrE mutants (#1–3) after 48 hours on BHIS (top left), BHIS supplemented with 10 μg.ml-1 Em (top right), C. difficile minimal media (CDMM) supplemented with uracil (bottom left) and CDMM supplemented with uracil and 5-flouroorotic acid (bottom right).