Table 2 List of plasmids utilised in this study.
Plasmid | Description | Source |
|---|---|---|
pMTL83151 | E. coli-clostridia shuttle vector with pCB102 replicon | Heap et al.19 |
pMTLcas-pta | CRISPR-Cas9 genome editing vector harbouring cas9 gene from Streptococcus pyogenes | Huang et al.14 |
pMTL431511-CLAU-pyrE | Modular CRISPR-Cas9 vector targeting C. autoethanogenum pyrE | This study |
pMTL431521-CDF-2008A | pMTL431511-CLAU-pyrE with TcdB promoter driving sgRNA expression, 2008A crRNA sequence and Tn5398 editing template | This study |
pMTL431521-CDF-2008B | pMTL431521-CDF-2008A with 2008B crRNA sequence | This study |
pMTL431521-CDF-2008C | pMTL431521-CDF-2008A with 2008C crRNA sequence | This study |
pMTL431521-CDF-pyrE1 | pMTL431521-CDF-2008A containing pyrE editing template and pyrE1 crRNA sequence | This study |
pMTL431521-CDF-pyrE2 | pMTL431521-CDF-pyrE1 with pyrE2 crRNA sequence | This study |
pMTL431521-CDF-pyrE3 | pMTL431521-CDF-pyrE1 with pyrE3 crRNA sequence | This study |
