Figure 4

Lactate dehydrogenase activity (LDH) in cell culture media of LN18 (A) and EJ28Luc (B) cells 48 h after 213Bi-anti-EGFR-MAb treatment. LDH, as determined with a commercial assay kit, did significantly differ in untreated controls and 213Bi-anti-EGFR-MAb treated cells, in EJ28Luc but not in LN18 cells. 213Bi-anti-EGFR-MAb treatment induced an elevated LDH activity in both cell lines indicative of cell death as LDH normally is stored intracellular but not in the extracellular compartment. Displayed are mean ± SD.