Figure 7

Effect of Ashwashila Treatment on Articular Cartilage Erosion of Knee Joint. (A) Histological analysis of normal control (NC) animal knee-joint stained with safranin ‘O’ show normal uncalcified cartilage (UC), calcified cartilage (CC), and subchondral bone (SB). (B) Knee-joint in disease control (DC) animal following treatment with C-Ab + LPS showed cartilage degradation extending up to SB. (C) Treatment of the diseased animal with Ashwashila (ASHW) limited the cartilage degradation till the UC region of the knee-joint. (D) Treatment of the diseased animal with methotrexate (MTX) showed superficial fibrillation of the articular cartilage (FB) region. (E) Increase in the pro-inflammatory lesion score was determined in the DC animals that showed reduction following treatment of the animals with ASHW or MTX. (F) Similar efficacy of ASHW and MTX in performing anti-arthritic activity was determined through a reduction in lesion score in the treated animals represented as percentage (%) inhibition. Values in the results are Mean ± SEM. A one-way analysis of variance (ANOVA) followed by Dunnett’s multiple comparison t-test was used to calculate the statistical difference. Student unpaired t-test was used to calculate statistical difference in comparison to MTX (p-value # ≤ 0.05; * ≤ 0.05; ** ≤ 0.01).