Figure 5 | Scientific Reports

Figure 5

From: Opposing effects of acute versus chronic inhibition of p53 on decitabine’s efficacy in myeloid neoplasms

Figure 5

Effects of acute and chronic p53 depletion on cell cycle and apoptosis in MLL-AF9 leukemia cells. (a–c) Cell-cycle status (a) and apoptosis (b) were assessed after 6 or 24 hours culture with vehicle or decitabine (50 nM), respectively. (a) FACS profiles of DMSO- or decitabine-treated MLL-AF9 cells are shown. The numbers indicate the percentages of cells in the G0/G1, S and G2/M phases. (b) FACS profiles of Annexin V and DAPI expression of DMSO- or decitabine-treated MLL-AF9 cells are shown. The numbers indicate the percentages of Annexin V+ and Annexin V+DAPI+ cells. (c) The frequency of G2/M phase cells in (a) (left) and that of Annexin V+ cells in (b) are shown as bar graphs.

Back to article page