Figure 6

Effects of acute and chronic p53 depletion on expression of p53 target genes in MLL-AF9 leukemia cells. Quantitative PCR of relative mRNA levels for several p53 target genes in MLL-AF9 cells with indicated p53 modulations. Cells were treated with DMSO or decitabine (50 nM) for 24 hours. Results were normalized to Gapdh, with the relative mRNA level in NT-transduced control cells was set to 1. Data are shown as mean ± s.d. of duplicate wells.