Figure 7 | Scientific Reports

Figure 7

From: Opposing effects of acute versus chronic inhibition of p53 on decitabine’s efficacy in myeloid neoplasms

Figure 7The alternative text for this image may have been generated using AI.

Effects of acute and chronic p53 depletion on differentiation and autophagy in MLL-AF9 leukemia cells. Immunophenotypes (a,b), morphology (c) and autophagy (d) were assessed after 24 hours culture with vehicle or decitabine (50 nM). (a) FACS profiles of Gr-1 and c-Kit expression of vehicle- or decitabine-treated MLL-AF9 cells with the indicated p53 modulations are shown. The numbers indicate the percentages of cells in each gate. (b) The frequencies of c-Kit+Gr-1− LSC-enriched population in each MLL-AF9 culture are shown as a bar graph. (c) Wright-Giemsa staining of cytospin preparations of each MLL-AF9 culture. Scale bars; 25 μm. (d) Levels of LC3 and Gapdh proteins in each MLL-AF9 culture. Band intensities of LC3-II relative to Gapdh are shown. The value of LC3-II/Gapdh in NT-transduced cells without decitabine treatment was set to 1.

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