Figure 7
Effects of acute and chronic p53 depletion on differentiation and autophagy in MLL-AF9 leukemia cells. Immunophenotypes (a,b), morphology (c) and autophagy (d) were assessed after 24 hours culture with vehicle or decitabine (50 nM). (a) FACS profiles of Gr-1 and c-Kit expression of vehicle- or decitabine-treated MLL-AF9 cells with the indicated p53 modulations are shown. The numbers indicate the percentages of cells in each gate. (b) The frequencies of c-Kit+Gr-1− LSC-enriched population in each MLL-AF9 culture are shown as a bar graph. (c) Wright-Giemsa staining of cytospin preparations of each MLL-AF9 culture. Scale bars; 25 μm. (d) Levels of LC3 and Gapdh proteins in each MLL-AF9 culture. Band intensities of LC3-II relative to Gapdh are shown. The value of LC3-II/Gapdh in NT-transduced cells without decitabine treatment was set to 1.
