Figure 5

In vitro release profiles of TBO from various conditions and antibacterial test of TBO formulations (TW60-F) with LED irradiation. Release of TBO from SP20-F and TW60-F formulations. (A) TBO formulations were applied on an acrylic plate and eluted in saline medium. The experiments proceeded in the dark room. TW60-F was gently stirred with magnetic bar at 150 rpm for 0 min, 15 min and 3 h in culture media, then added to the culture wells to give final TBO concentrations of 0.33 mM. After 5 min incubation, (B,D) P. gingivalis and (C,E) F. nucleatum bacterial suspension wells were exposed to 60 mW/cm² of LED at 650 nm wavelength for 1 min and 5 min. After irradiation, all the suspended bacteria was spread on an agar plate and incubated at 37 °C for 48–72 h. Then the viable bacterial colonies were counted. The data represent mean ± SD. (n = 2). *p < 0.05 vs. control (without TW60_F, without LED irradiation).