Figure 3

Enzyme kinetic parameters of microsomal in vitro AFB1-dhd production. (a) Saturation curve at AFB1 concentrations of 13.9 to 256 μM (incubations with duck microsomal fraction were done at AFB1 concentrations of 1.23 to 22.6 μM). (b) Maximal velocity (Vmax). (c) Michaelis-Menten constant (KM). (d) Intrinsic clearance (CLint; Vmax/KM). Total mean values with the same letter do not differ significantly. (*) Statistical differences by sex (P < 0.05) were calculated by the Kruskal-Wallis test.