Figure 2
From: Dysregulated autophagy in muscle precursor cells from humans with type 2 diabetes
DRAM1, VAMP8 and TP53INP1 expression in myoblast derived from humans with T2DM and healthy control subjects. (A) Gene counts from 21for identified differentially expressed autophagy-related genes (adjusted p-value < 0.5) in T2DM subjects (n = 6) versus healthy controls (n = 6). (B) Myoblasts from healthy controls or T2DM donors were harvested under proliferation, at confluence (day -2), as aligned, undifferentiated myoblasts (day 0), at early differentiation, after 2 days in differentiation medium (day 2) and as fully differentiated moytubes, after 5 days in differentiation medium (day 5). DRAM1, VAMP8 and TP53INP1 mRNA was measured by RT-qPCR in healthy or T2DM muscle precursor cells. mRNA expression was normalized to the geometric mean of 18 s, B2M and PPIA mRNA. Data are means ± SEM. * indicates P < 0.05; **P < 0.01.
