Figure 1
From: Plasmodium pseudo-Tyrosine Kinase-like binds PP1 and SERA5 and is exported to host erythrocytes
In silico annotation of PF3D7_1106800 (PfpTKL) catalytic domains. (a) PfpTKL has a pseudo-kinase domain. Sequence alignment between human PKAc (used as the kinase domain reference), RAF1 (used as a TKL reference), RIPK1 (used as a pseudo-TKL reference), BTK (used as a tyrosine kinase reference) and PfpTKL kinase domains. The kinase subdomains (I to XI on the sequence alignment) were delineated according to Hanks & Hunter23 and are shaded. Non canonical S/T kinase subdomain amino acids are shown in red and marked with a red star. The activation loop residues are shown in dark blue. (b) PfpTKL possesses a Bruton’s tyrosine kinase (BTK) N-terminal lobe-like domain upstream of its pseudo-kinase domain. Protein sequence alignment of BTK-like domains of PfpTKL and its Plasmodium homologs with the N-terminal lobe sequences of human BTK and PKAc. The alignment was created with MAFFT89 and conserved residues were shaded using BioEdit v7.2.5116. The ATP-binding lysine conserved across all the aligned sequences is indicated with a red arrow.
