Figure 4
Peli1 mediates LPS-induce inflammatory responses in microglia. (a) QPCR analysis of relative mRNA expression for the indicated inflammatory genes in the primary cultured microglia isolated from WT and Peli1-KO mice, left untreated (NT) or stimulated with LPS (100 ng/ml). (b) ELISA of IL-1β, IL-6, TNF and nitrites in the supernatant of WT and Peli1-KO microglia cultures that left untreated (NT) or stimulated with LPS (100 ng/ml) at the indicated time points. (c,d) QPCR analysis of relative mRNA expression for the indicated inflammatory genes in the primary cultured microglia (c) or astrocytes (d) isolated from WT and Peli1-KO mice, left untreated (NT) or stimulated with IL-1β (20 ng/ml). The gene expression were normalized to a reference gene Actb (encoding β-actin) and showed as the relative value to Actb in bar graphs. Data with error bars represent mean ± SD. Each panel is representative of at least three independent experiment. *P < 0.05, **P < 0.01 as determined by two-way ANOVA with a Bonferroni post test.
