Figure 5 | Scientific Reports

Figure 5

From: Combination of TLR2 and TLR3 agonists derepress infectious bursal disease virus vaccine-induced immunosuppression in the chicken

Figure 5

Infectious bronchitis virus (IBV)-specific antibody titers in specific pathogen-free chickens. Birds were immunized at 4 weeks of age by ocular instillation of live intermediate plus IBDV vaccine and intramuscular injection of Pam3CSK4 and/or poly I:C. All the birds (n = 7/group) were immunized with live IBV vaccine through ocular instillation at 21 dpi, with a booster given 14 days later. The positive control group included three birds receiving the IBV vaccine strain without prior IBDV vaccination whereas birds in the negative control group (n = 3) were administered PBS through the intramuscular route. Humoral immune responses against IBV (n = 6/group; except for the negative and positive control groups, with n = 3/group) were measured using a commercial IDEXX ELISA kit at weekly intervals until 56 dpi. Treatment effects were analyzed by one-way analysis of variance. The probability of an alpha error was set at 0.05. Different superscripts above bars (mean ± SE) indicate statistically significant differences (P < 0.05). dpi: days post-IBDV immunization. *IBV specific antibody titers greater than 396 were considered positive responses as recommended by the ELISA kit.

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