Figure 4 | Scientific Reports

Figure 4

From: Automated detection of the HER2 gene amplification status in Fluorescence in situ hybridization images for the diagnostics of cancer tissues

Figure 4

Application of the deep learning-based system on six interphase nuclei detection. The signal detector (SD) detects FISH signals in each of the separated and pre-classified nuclei from the nucleus detector (ND) and classifies the signals into HER2 (light blue framed boxes), HER2 cluster (dark blue framed boxes) and CEN17 (red framed boxes) signals. Exemplarily three nuclei are shown: (A–C) The classification was confirmed. (D) The classification was not confirmed due to a misinterpretation of three very adjacent HER2 gene single signals as HER2 cluster. (E) The classification was not confirmed because two HER2 gene signals were not detected by the signal detector. (F) The classification was not confirmed although all FISH signals were detected by signal detector because the classification was defined to be unclassifiable if only one CEN17 signals occurs.

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