Table 4 List of F. circinatum diagnostic protocols assessed during the collaborative study.

From: Transferability of PCR-based diagnostic protocols: An international collaborative case study assessing protocols targeting the quarantine pine pathogen Fusarium circinatum

Protocol number

Reference

Target in the F. circinatum genome

Type of assay

p1

Ramsfield et al.23

Two independent sequence characterized amplified regions (SCAR)

End-point PCR

p2

Ioos et al.24

rDNA Intergenic spacer (IGS)

Real-time PCR with hydrolysis probe

p3

Lamarche et al.27

rDNA Intergenic spacer (IGS)

Real-time PCR with hydrolysis probe

p4

Schweigkofler et al.22

rDNA Intergenic spacer (IGS)

Real-time PCR with SYBR Green staining

p5

Fourie et al.26

rDNA Intergenic spacer (IGS)

Real-time PCR with SYBR Green staining

p6

Dreaden et al.25

rDNA Intergenic spacer (IGS)

Real-time PCR with SYBR Green staining

p7

Luchi et al.28

Translation elongation factor 1-alpha gene (TEF)

Real-time PCR with hydrolysis probe

p8

Baskarathevan et al. (Supplementary Information 1)

Sequence characterized amplified region (SCAR)

Real-time PCR with hydrolysis probe

p9

EPPO13, appendix 4

rDNA Intergenic spacer (IGS)

End-point PCR