Table 4 List of F. circinatum diagnostic protocols assessed during the collaborative study.
Protocol number | Reference | Target in the F. circinatum genome | Type of assay |
|---|---|---|---|
p1 | Ramsfield et al.23 | Two independent sequence characterized amplified regions (SCAR) | End-point PCR |
p2 | Ioos et al.24 | rDNA Intergenic spacer (IGS) | Real-time PCR with hydrolysis probe |
p3 | Lamarche et al.27 | rDNA Intergenic spacer (IGS) | Real-time PCR with hydrolysis probe |
p4 | Schweigkofler et al.22 | rDNA Intergenic spacer (IGS) | Real-time PCR with SYBR Green staining |
p5 | Fourie et al.26 | rDNA Intergenic spacer (IGS) | Real-time PCR with SYBR Green staining |
p6 | Dreaden et al.25 | rDNA Intergenic spacer (IGS) | Real-time PCR with SYBR Green staining |
p7 | Luchi et al.28 | Translation elongation factor 1-alpha gene (TEF) | Real-time PCR with hydrolysis probe |
p8 | Baskarathevan et al. (Supplementary Information 1) | Sequence characterized amplified region (SCAR) | Real-time PCR with hydrolysis probe |
p9 | EPPO13, appendix 4 | rDNA Intergenic spacer (IGS) | End-point PCR |