Table 1 Comparison of lymphocytes isolated using various methods.
Method | Flow rate (μL/min) | Throughput (cells/min) | Purity (%) | Total time (min) | Cost | Reference |
|---|---|---|---|---|---|---|
Traditional | ||||||
Surface modification | ~10 | ~1.6*105 | ~95 | ~90 | High | |
Cell adhesion | ~50 | ~2.5*105 | ~60 | ~40 | Low | |
Density gradient | ~167 | ~2*105 | ~90 | ~70 | High | |
Microfluidic | ||||||
Immunomagnetic | ~83.3 | ~2.9*103 | ~95 | ~170 | High | |
Hydrodynamic filtration | ~10 | ~6*103 | ~90 | ~15 | High | |
Deterministic Lateral Displacement | ~0.2 | ~6*104 | ~90 | ~40 | Low | |
Step pattern filtration | ~0.1 | ~7.7*103 | ~90 | ~60 | Low | |
Dean drag force & Inertial lift force | ~1700 | ~1.3*105 | 97 ± 2 | ~15 | Low | our device |
