Figure 10

Widespread reduction of myelin, and axonal density in the hippocampus during experimental autoimmune encephalomyelitis (EAE) mice at long-term disease duration (66 days). Neuronal nucleus staining (NeuN - red) was not reduced in EAE mice (C) compared to Naïve (A) or CFA (B) controls. Quantification of neuron density depicted in graph (D). Myelin basic staining (MBP - green) was significantly reduced in EAE mice (G) compared to Naïve (E) or CFA (F) controls. Quantification of percent area of MBP pixel intensity depicted in graph (H). Axon staining (SMI-312 - red) was significantly reduced in EAE mice (K) compared to Naïve (I) or CFA (G) controls. Quantification of percent area of SMI-312 pixel intensity depicted in graph (K). DAPI – blue. n = 5 Naïve, 5 CFA, 10 EAE (NeuN), 5 EAE (SMI-312/MBP), *p < 0.05, **p < 0.01, ***p < 0.001, ANOVA test with Tukey post-hoc.