Figure 4

High score EAE mice have significantly smaller brains at long-term disease (day 66+). (A) Cerebellum volume (cerebellar cortex + arbor vita of cerebellum + cerebellar peduncle: inferior, middle, superior) in Naive, CFA, Low Score EAE and High Score EAE mice. EAE mice were divided into two groups based on cumulative long-term disease scores (summed disease scores days 30–66). Mice with below average scores were grouped as Low Score EAE and mice with above average scores were grouped as High Score EAE. (B) Hippocampal volume (C) Corpus callosal volume. (D) Cerebral cortex (CTX) volume (frontal lobe + parieto-temporal lobe + occipital lobe + entorhinal cortex). All volumes are adjusted based on weight using an analysis of covariance (ANCOVA). n = 9 Naïve, 13 CFA, 27 EAE, 12 Low Score EAE, 15 High Score EAE. *q < 0.05; **q < 0.01 ***q < 0.001. q values represent p values corrected for body weight and multiple comparisons via the false discovery rate (FDR) method.