Figure 2
From: Hes1 expression in mature neurons in the adult mouse brain is required for normal behaviors
Induction of Hes1 expression by neuronal stimulation. (a) Immunofluorescence staining for Hes1 (left panels), Arc (middle panels), and c-Fos (right panels) in the primary visual cortex of wild-type mice before (upper panels) and 2 hours after (lower panels) visual stimulation. (b) Double immunofluorescence staining for GFP (left panels), NeuN (middle panels), and merged images (right panels) in the primary sensory cortex of Venus-Hes1 knock-in mice before (upper panels) and 2 hours after (middle panels) electroconvulsive stimulation. Wild-type (WT) mice were shown as a negative control of immunostaining for GFP (lower panels). Cortical layers I-VI are labeled on the right. Scale bars = 100 μm.
