Figure 4
From: Hes1 expression in mature neurons in the adult mouse brain is required for normal behaviors
Cell excitability in the amygdala differs in excitatory neuron-specific Hes1 cKO mice. (a) Double immunofluorescence staining for c-Fos (green) and NeuN (blue) in the medial pre-frontal cortex (mPFC, upper panels) and basolateral amygdala (BLA, lower panels) of wild-type control and Math2-Cre;Hes1 cKO mice. We first performed fear conditioning experiment, and then examined c-Fos expression 2 hours after the second cued recall test. Scale bars = 100 µm. (b) Bar graphs showing the density of c-Fos-positive neurons in mPFC and BLA of wild-type control (black, n = 4) and Math2-Cre;Hes1 cKO (gray, n = 4) mice. Data are presented as mean ± SEM; *p < 0.05; two tailed Student’s t-test. (c) Double immunofluorescence staining for Hes1 (green) and NeuN (blue) in the BLA of wild-type control and Hes1 cKO mice 2 hours after the auditory cue. Scale bar = 100 µm.
