Figure 2
The conditional knockout of Ash2l interferes with adult hematopoiesis. (a) Whole blood from control (n = 49) and Ash2lfl/fl: Mx1-Cre KO (n = 35) mice was analyzed. WBC, white blood cells; PLT, platelets; RBC, erythrocytes; HGB, hemoglobin; HCT, hematocrit. (b) Blood count of lymphocytes, granulocytes, and monocytes from control (n = 7) and Ash2l KO (n = 6) mice. (c) Erythrocyte parameters from control and KO mice as in (panel a). MCV, mean corpuscular volume; MCH, mean corpuscular hemoglobin; MCHC, mean corpuscular hemoglobin concentration. (d) Flow cytometry analysis of BM cells from control (n = 3) and KO (n = 5) mice. Cells were labeled with lineage specific antibodies as indicated: CD3, T cells; CD4, helper T cells; CD8, cytotoxic T cells; B220, B cells; Gr1, granulocytes; CD11b, granulocytes/monocytes; Ter119, erythrocytes; CD41, megakaryocytes/platelets and myelo-erythoid progenitors; CD117, c-Kit positive cells. (e) Flow cytometry analysis of spleen cells from control (n = 3) and pIC treated KO (n = 5) mice as in (panel d). (****p < 0.0001; ***p < 0.001; **p < 0.01; *p < 0.05; ns, not significant).
