Figure 4
The loss of Ash2l protein expression deregulates gene expression. (a) Total RNA of LSK cells (n = 3 each) was analyzed on microarrays. A volcano plot was employed to visualize differences in gene expression. (b) RT-qPCR analysis of selected genes from (a) using RNA prepared from LSK cells of biological replicates. (c,d) GO analysis of down- and up-regulated genes (panels c and d, respectively) with the indicated p-values (circle size). The semantic space represents the similarities of GO terms, i.e. GO terms annotated with similar genes have similar values. Only representative GO terms are shown.
