Figure 6
No increase in apoptosis in Ash2l−/− cells. (a) Annexin V staining of lineage negative (lin−) cells isolated from BM of control and KO animals (n = 4 each). (b) Sections of fixed sterna from Ash2l KO animals stained with H&E and with TUNEL as indicated. The top panels show apoptotic megakaryocytes, the middle panels apoptotic granulocytes (circles). The bottom panel summarizes TUNEL positive cells from 10 HPFs of 2 control and 2 KO animals, corrected for the number of cells present in each section. (c) Sections of fixed sterna from Ash2l KO animals stained with H&E and immunohistochemically for F4/80 as indicated. Circles indicate macrophages. (d) Complementation with human ASH2L. Lin− cells (n = 3 each) were infected with control or hASH2L-expressing virus, seeded in semi-solid media and the number of colonies was counted. (****p < 0.0001; **p < 0.01; ns, not significant).
