Figure 7
Physicochemical analyses of oligomeric states and molecular shapes of Rif1 polypeptides. Upper: Size-exclusion (gel-filtration) patterns of the partially purified fraction of His6-Rif1-FLAG3 (containing a ~70 kDa degradation product derived from the C-terminal segment). Retention volume of the molecular size standard was determined by monitoring A260. Lower: Sedimentation pattern of the same fraction through glycerol-gradient centrifugation. The three peaks of the full-length Rif1 are marked as F, M and S in order of sedimentation speed. The four peaks of the ~70 kDa polypeptide are marked similarly as F, M2, M1 and S. Sedimentation pattern of molecular size standards was analyzed by SDS-PAGE followed by silver-staining (data not shown). In both panels, the positions of size markers and those of the full-length and degradation polypeptide are shown by red, green and blue filled arrowheads, respectively.
