Figure 3

Adenovirus Expression of Fluorescent Protein Biosensors in Islets Transplanted under the Mouse Kidney Capsule. (a) Top left: Schematic depiction of IVM of biosensor-labelled isolated primary mouse islets transplanted under the mouse kidney capsule after adenoviral transduction in vitro. Right: Representative image of an islet graft under the kidney capsule labeled with Grx1-roGFP2. (b) Fold change over baseline in β-cell-specific Grx1-roGFP2 from 3 mice, 5 minutes after IV saline injection, and 5 and 15 minutes after IV injection of 80 mg/kg alloxan monohydrate. Pairwise t-tests were calculated for each time point between groups. Data are means ± SEM (N = 3, *p < 0.05). (c) Representative 405/488 ratiometric images of Grx1-roGPF2 changes in β-cells within the islet transplant under the kidney capsule at baseline, 5 minutes after IV saline injection, and 5 and 15 minutes after IV injection of 80 mg/kg alloxan monohydrate.