Figure 1

Leaf shape of rpt5a-4 under zinc deficiency. (A) Fourteen-day-old seedlings of wild type, rpt5a-4, and rpt5a-4 complementation line grown under control and zinc deficiency conditions. Bar; 5 mm. (B–E) Leaf shapes were categorized into four groups, round tip (B), edged tip (C), narrow (D), and filamentous (E), as illustrated in (B) from left to right. Bar; 5 mm. (F) Frequency was calculated from observation of at least 20 individual plants for each condition. The numbers with “#” are identifiers for independent transformant lines in the complementation experiment. There is no significant difference in the ratio of (“round tip” + “edged tip”): (“narrow” + “filamentous”) at p < 0.05 among groups sharing the same letter by Fisher’s exact test with Holm correction.