Figure 2
From: SNAI1 recruits HDAC1 to suppress SNAI2 transcription during epithelial to mesenchymal transition
SNAI1 overexpression leads to downregulation of SNAI2. (a) Phase contrast images showing morphology of OVCA429 cells stably expressing control (EV) and full-length SNAI1 (SNAI1). Bottom panels showing immunofluorescence staining of E-cadherin and Vimentin in control and SNAI1 overexpressing cells. Nuclei were stained blue (DAPI), Scale = 50 μm. (b) Immunoblots showing expression of SNAI1 & SNAI2 in control and SNAI1 overexpressing cells. α-Tubulin was used as a loading control. (c) Phase contrast images of OVCA429 cells expressing control and SNAI1 cells at different time points after the addition of doxycycline (2 μg/ml), Scale = 50 μm. (d,e) Expression levels of indicated genes upon different time points after doxycycline treatment as analysed by qRT-PCR (d) and western blotting (e).
