Figure 1
Analysis of insulator effect over LUC activity. (A) Schematic representation of the constructs used for studying the effect genomic insulators flanking transgenes. The above scheme represents the construct used as a control (LUC) while the scheme below represents the four constructs flanked by the four different insulators. pMAS: mannopine synthase gene promoter; LUC: firefly luciferase; Tnos: nopaline synthase terminator: pNos: nopaline synthase promoter; Tocs: octopine synthase terminator. “Insulator” represents Rb7, chiMAR, TBS or AtS/MAR10. (B) Time course of LUC activity when expressed under the pMAS promoter. Lines were assayed for LUC imaging at 12, 22 and 28 days-old. Results for control line LUC 14.9 (indicated with an arrow) are shown, but similar data was obtained for the rest of the lines. d.o.s: day-old seedlings; cps: counts per second. (C) Box and whisker plots showing LUC activity. ** Represents Student’s test significant differences (p < 0.005); *** represents Student’s test highly significant differences (p < 0.001); cps: counts per second. (D) LUC activity imaging of the T3 homozygous lines, eight lines per construct.
