Figure 4 | Scientific Reports

Figure 4

From: Benefits of using genomic insulators flanking transgenes to increase expression and avoid positional effects

Figure 4

Analysis of the DNA methylation levels in the junction between the host genome and the T-DNA for line chiMAR 6.13. Upper panel: schematic representation of the junction site. Middle panel: graphical output of the methylation analysis (CyMate software) in 12 day-old seedlings of chiMARs 6.13 line. Red circles represent CG sites, blue squares represent CHG sites and green triangles represent CHH sites. Filled symbols indicate methylated cytosines while empty ones represent non methylated cytosines. Lower panel: the graph shows the DNA methylation quantification of CG (red bars), CHG (blue bars) and CHH (green bars) cytosine contexts for the flanking sequence (left) and the T-DNA (right).

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