Table 1 Biochemical assessment of mitochondrial function in epididymal adipose tissue explants from the vicinity of implanted FGF8b release or placebo pellets.

From: Fibroblast growth factor 8b induces uncoupling protein 1 expression in epididymal white preadipocytes

 

Placebo

Fgf8b

p

1

adipocyte respiration on G3P (nmol O2/s*g tissue)

0.84 ± 0.30

0.86 ± 0.36

0.923

2

cytochrome C oxidase activity (nmol O2/s*g tissue)

3.93 ± 1.64

5.24 ± 2.36

0.289

3

citrate synthase activity (arbitrary unit/g tissue)

1.12 ± 0.51

1.36 ± 0.41

0.396

4

ratio of 1 to 2

0.22 ± 0.04

0.17 ± 0.02

0.016

5

ratio of 1 to 3

0.82 ± 0.31

0.63 ± 0.20

0.244

6

ratio of 2 to 3

3.74 ± 1.42

3.81 ± 1.23

0.935

  1. 1 - Respirometry of isolated, mature adipocytes with glycerol-3-phosphate (G3P) as substrate. 2–Cytochrome C oxidase activity in tissue homogenates. 3 – Citrate synthase activity in tissue homogenates. 4–6 – Ratios between 1–3. None of the parameters assessed was significantly different (Sidak’s multiple comparisons test), n = 6.
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