Figure 5

Baseline pulmonary protein expression of key components of TLR4 signaling is not different between neonatal male and female mice. (A) Representative Western blots and (B) densitometric analysis showing male (white bars) and female (black bars) neonatal (P0) pulmonary expression of (A,B) key components of TLR4 signaling: TLR4 and Myd88; (A,C) NFκB activating kinases: IKKα and IKKβ; (A,D) NFκB inhibitory proteins: IκBα, IκBβ; and (A,E) NFκB subunits: p50, p65 and cRel. GAPDH as loading control. Values first normalized to GAPDH, and then to male control. Values shown as means ± SEM from 6 individual animals per sex.