Figure 2

Treatment with oATP suppresses CD4⁺ effector T cell generation and graft infiltration. (A) Representative flow cytometric dot plots (left) and cumulative bar chart (right) showing the frequencies of CD4⁺IFNγ⁺ cells (gated on CD4⁺ cells) in the draining lymph nodes of oATP-treated mice, compared to saline-treated and naïve mice. (B) Representative flow cytometric dot plots (left) showing the gating strategy for selecting CD4⁺ cells in the cornea. Bar chart (right) summarizes the frequencies of CD4⁺ cells in the cornea at 2 weeks post-transplantation, relative to saline-treated and naïve mice. n = 5–7/group. Representative data from three independent experiments are shown, and data are depicted as mean ± SEM. *p < 0.05; **p < 0.01.