Figure 3
Histological analysis of growth plates of mice treated for 3 and 10 days. (a,e) Alcian-blue staining of growth plates of mice treated for (a) 3 and (e) 10 days. Yellow and red bars indicate the widths of the proliferative and hypertrophic zones, respectively. Black scale bar indicates 100 µm. (b,f) The widths of whole growth plates measured on histological images of alcian-blue staining after treatment for (b) 3 and (f) 10 days. (c,g) The widths of hypertrophic zones measured on histological images of alcian-blue staining after treatment for (c) 3 and (g) 10 days. (d,h) The widths of proliferative zones measured on histological images of alcian-blue staining after treatment for (d) 3 and (h) 10 days. (b–d,f–h) n = 3, each, in the CNP/vehicle, vehicle/vehicle, vehicle/DEX, and CNP/DEX groups. *P < 0.05, vs. vehicle/vehicle and †P < 0.05, vs. vehicle/DEX.
