Figure 4

The effect of CNP-53 administration from 5 weeks of age. (a) Growth curves and (b) growth velocities of naso-anal lengths of CNP/vehicle (●), vehicle/vehicle (○), vehicle/DEX (Δ), and CNP/DEX (▲). (c) Bone lengths after treatment for 4 weeks (9 weeks old). (d,e) The mRNA levels of Nr3c1 which encodes the murine glucocorticoid receptor normalized by using Hprt as the reference gene. (d) Lumbar vertebrae and (e) costal cartilages. The data are represented as fold-change versus the values for 4-week-old mice. (a–c) CNP/vehicle, n = 5; vehicle/vehicle, n = 4; vehicle/DEX, n = 4; and CNP/DEX, n = 4, and (d–e) n = 3, each, in 4-week-old and 5-week-old mice. (a–c) *P < 0.05, vs. vehicle/vehicle and ^†P < 0.05, vs. vehicle/DEX. (d,e) ^‡P < 0.05.