Figure 1

Gating strategy performed by flow cytometry. (a) PBMC were identified based on side scatter (SSC) and forward scatter (FSC). (b) CD4⁺ T cells were identified from PBMC based on CD4 expression. (c) Gating strategy for CD45RO⁺ memory Th cells (upper panel) and FOXP3⁺CD25⁺CD4⁺ Treg cells (lower panel), respectively. (d) CD4⁺ CD45RO⁺ memory Th cells and FOXP3⁺CD25⁺CD4⁺ Treg cells, respectively, were gated as CCR4⁺CCR7⁻ and CCR7⁺ subset. (e) Gating strategy for the cells which express sialyl Le^X, sialyl 6-sulfo Le^X, or cyclic sialyl 6-sulfo Le^X.