Figure 4

Relative decapping efficiency of yDcpS with 30 different cap structures on 25mer RNAs. (a) chemical representation of cap structures. (b) Composite of gel images of 6 decapping reactions of 25mer RNA substrates (m⁷Gppp⁶mA, m⁷GpppG, m⁷GpppGm, GpppG, m⁷Gppp⁶mAm, m⁷GpppA) incubated with increasing concentrations of yDcpS for 60 minutes at 37 °C. The reactions were electrophoresed on a 15% TBE-Urea gel and stained with SYBR Gold. The full-length gel images are shown in the Supplemental Information. (c) Substrates decapped (greater than 95% at 2.7 µM yDcpS) are shaded in green and substrates resistant to decapping (less than 10% at 25 µM yDcpS) are shaded in pink. Bolded characters indicate canonical cap structures (known or anticipated).