Figure 3
DNA methylation levels in the spermatozoa and testis of 40-week-old male chickens. Total DNA methylation levels in the sequenced regions of (a) ATP5A1, AMPKα2, and mTOR in the spermatozoa and (b) STAR, CYP11A1, CYP17A1, and AR in the testis. Total methylation ratios were calculated by dividing the number of non-converted (methylated) cytosines by the total number of cytosines within the sequenced region; values were expressed as percentages (%). Average methylation levels for CG, CHG, and CHH in the sequenced regions of (c) ATP5A1, (d) AMPKα2, and (e) mTOR in the spermatozoa, (f) STAR, (g) CYP11A1, (h) CYP17A1, and (i) AR in the testis. Average methylation levels were expressed as percentage (%) per site for each of the three types of cytosines (CG, CHG, and CHH), and were calculated by dividing the number of non-converted cytosines by the total number of cytosines of each type. The cytosine methylation analysis results see Supplementary Fig. S3. An independent replicate on DNA methylation levels in the spermatozoa and testis of 40-week-old male chickens see Supplementary Fig. S5.
