Table 1 Key microRNAs (miRNAs) with differential expression in the testis of 40-week-old male chickens.

From: Demethylation and microRNA differential expression regulate plasma-induced improvement of chicken sperm quality

MiRNA name

Sequence

Sequence cordinate

P/C. FC

Associated target genes

gga-miR-7450-5p

UCUGUUCUUAAGGAGGCUGAGGC

chr1:54894245-54894267 (+)

1.81568

AMPK

gga-miR-2954

CAUCCCCAUUCCACUCCUAGCA

chrZ:70557207-70557228 (+)

1.543874

AMPK

gga-miR-130a-3p

CAGUGCAAUAUUAAAAGGGCAU

chr15:399369-399390 (−)

−1.606146

PRDX3, HSD17B3

gga-miR-222a

AGCUACAUCUGGCUACUGGGUCUC

chr1:109977233-109977256 (+)

−1.949927

ATP5L

gga-miR-30d

UGUAAACAUCCCCGACUGGAAG

chr2:142229538-142229559 (−)

−2.298415

ATP5G1

gga-miR-181b-5p

AACAUUCAUUGCUGUCGGUGGG

chr17:9498558-9498579 (+) // chr8:1986890-1986911 (+)

−3.837102

PRDX3

gga-miR-15b-5p

UAGCAGCACAUCAUGGUUUGCA

chr9:21653636-21653657 (−)

−7.086145

ATP5C1, ATP5L, ATP5S

gga-miR-23b-3p

AUCACAUUGCCAGGGAUUACC

chrZ:41508087-41508107 (+)

−7.314428

ATP5G1

gga-miR-99a-5p

AACCCGUAGAUCCGAUCUUGUG

chr1:98347485-98347506 (+)

−7.925797

MTOR

gga-miR-100-5p

AACCCGUAGAUCCGAACUUGUG

chr24:3330849-3330870 (+)

−8.741549

MTOR

gga-miR-130b-3p

CAGUGCAAUAAUGAAAGGGCGU

chr15:390547-390568 (−)

−9.207497

PRDX1, HSD17B3

gga-miR-456-3p

CAGGCUGGUUAGAUGGUUGUCA

chr3:30789084-30789105 (−)

−10.561713

ATP5S

gga-miR-221-3p

AGCUACAUUGUCUGCUGGGUUUC

chr1:109977744-109977766 (+)

−11.892606

ATP5L

gga-miR-107-3p

AGCAGCAUUGUACAGGGCUAUCA

chr6:18945422-18945444 (−)

−12.796044

ATP5S

gga-let-7b

UGAGGUAGUAGGUUGUGUGGUU

chr1:71371984-71372005 (+)

−13.241843

PRDX1, PRDX4

gga-miR-26a-5p

UUCAAGUAAUCCAGGAUAGGC

chr2:4537775-4537795 (+)

−13.680907

PRDX1

gga-miR-103-3p

AGCAGCAUUGUACAGGGCUAUGA

chr13:4060451-4060473 (+)// chr4:88047793-88047815 (−)

−14.044584

ATP5S

gga-miR-24-3p

UGGCUCAGUUCAGCAGGAACAG

chrZ:41508848-41508869 (+)

−19.129192

ATP5L

gga-miR-16-5p

UAGCAGCACGUAAAUAUUGGUG

chr1:168694597-168694618 (−)// chr9:21653466-21653487 (−)

−33.39268

ATP5C1

gga-miR-27b-3p

UUCACAGUGGCUAAGUUCUGC

chrZ:41508332-41508352 (+)

−42.958092

PRDX3

gga-miR-106-5p

AAAAGUGCUUACAGUGCAGGUA

chr4:3946676-3946697 (−)

−63.056273

STAR

  1. P, plasma treatment; C, control; FC, fold change. miRNA with |FC| ≥ 1.5 and a p-value < 0.05 is significantly up- or down-regulated. All differentially expressed miRNAs following the plasma treatment are shown in Supplementary Table S1.
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